tissue antigen recovery Search Results


human embryonic kidney hek 293t  (ATCC)
99
ATCC human embryonic kidney hek 293t
The O-GlcNAcylation of eIF2α downregulates the antioxidant capacity, cell recovery and migratory ability of breast cancer cells in response to ArgS. A Immunoprecipitation coupled with immunoblot analysis was performed to measure the levels of GlcNAz-modified (O-GlcNAcylated) proteins in parental <t>HEK</t> <t>293T</t> cells (n = 3; left panel ). The O-GlcNAz-modified eIF2α level was calculated by normalizing the densitometric tracing of eIF2α signal to the H3 signal ( right panel ). The values were quantified using ImageLAB software and adjusted for background. B The same analysis was performed in WT and O-GlcN-mut, eIF2α overexpressing HEK <t>293</t> <t>T</t> cells (n = 2; left panel ). The O-GlcNAz-modified eIF2α level was calculated as described in A , and the ratio in each experimental condition was compared to the reference (WT + Arg, set to 1; left panel ). One representative immunoblot is shown for each analysis, with indicated antibodies. The black arrowheads indicate endogenous eIF2α, and the white arrowheads indicate FLAG-tagged eIF2α. C The levels of ROS were measured in BT-549 cells stably expressing WT or O-GlcN-mut eIF2α after 48 h of ArgS treatment (n = 3). The ROS levels were quantified using CellROX™ and flow cytometry. D The relative cell recovery was monitored in BT-549 cells stably overexpressing WT or O-GlcN-mut eIF2α over a 5-day period, after 24 h of ArgS treatment and replacement with complete medium. The cell recovery was determined using ACP assays and the data were normalized to the values of Day 0, set to 1. E The cell migration was measured in BT-549 cells stably overexpressing WT or O-GlcN-mut eIF2α after 24 h of ArgS treatment and replacement with complete medium. The cell migration was determined by calculating the % confluency within the gap area and was imaged and quantified using Image J software at 0, 12, 24, and 30 h. Data are presented as mean ± s.e.m. and analyzed using Two-Way ANOVA followed by Tukey's multiple comparison test. Statistical significance was determined with * p < 0.05; ** p < 0.01; *** p < 0.001
Human Embryonic Kidney Hek 293t, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tissue antigen recovery  (Vector Laboratories)
96
Vector Laboratories tissue antigen recovery
The O-GlcNAcylation of eIF2α downregulates the antioxidant capacity, cell recovery and migratory ability of breast cancer cells in response to ArgS. A Immunoprecipitation coupled with immunoblot analysis was performed to measure the levels of GlcNAz-modified (O-GlcNAcylated) proteins in parental <t>HEK</t> <t>293T</t> cells (n = 3; left panel ). The O-GlcNAz-modified eIF2α level was calculated by normalizing the densitometric tracing of eIF2α signal to the H3 signal ( right panel ). The values were quantified using ImageLAB software and adjusted for background. B The same analysis was performed in WT and O-GlcN-mut, eIF2α overexpressing HEK <t>293</t> <t>T</t> cells (n = 2; left panel ). The O-GlcNAz-modified eIF2α level was calculated as described in A , and the ratio in each experimental condition was compared to the reference (WT + Arg, set to 1; left panel ). One representative immunoblot is shown for each analysis, with indicated antibodies. The black arrowheads indicate endogenous eIF2α, and the white arrowheads indicate FLAG-tagged eIF2α. C The levels of ROS were measured in BT-549 cells stably expressing WT or O-GlcN-mut eIF2α after 48 h of ArgS treatment (n = 3). The ROS levels were quantified using CellROX™ and flow cytometry. D The relative cell recovery was monitored in BT-549 cells stably overexpressing WT or O-GlcN-mut eIF2α over a 5-day period, after 24 h of ArgS treatment and replacement with complete medium. The cell recovery was determined using ACP assays and the data were normalized to the values of Day 0, set to 1. E The cell migration was measured in BT-549 cells stably overexpressing WT or O-GlcN-mut eIF2α after 24 h of ArgS treatment and replacement with complete medium. The cell migration was determined by calculating the % confluency within the gap area and was imaged and quantified using Image J software at 0, 12, 24, and 30 h. Data are presented as mean ± s.e.m. and analyzed using Two-Way ANOVA followed by Tukey's multiple comparison test. Statistical significance was determined with * p < 0.05; ** p < 0.01; *** p < 0.001
Tissue Antigen Recovery, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tissue+antigen+recovery/pm39133811-36-5-9?v=Vector+Laboratories
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antigen retrieval buffer  (Abcam)
95
Abcam antigen retrieval buffer
The O-GlcNAcylation of eIF2α downregulates the antioxidant capacity, cell recovery and migratory ability of breast cancer cells in response to ArgS. A Immunoprecipitation coupled with immunoblot analysis was performed to measure the levels of GlcNAz-modified (O-GlcNAcylated) proteins in parental <t>HEK</t> <t>293T</t> cells (n = 3; left panel ). The O-GlcNAz-modified eIF2α level was calculated by normalizing the densitometric tracing of eIF2α signal to the H3 signal ( right panel ). The values were quantified using ImageLAB software and adjusted for background. B The same analysis was performed in WT and O-GlcN-mut, eIF2α overexpressing HEK <t>293</t> <t>T</t> cells (n = 2; left panel ). The O-GlcNAz-modified eIF2α level was calculated as described in A , and the ratio in each experimental condition was compared to the reference (WT + Arg, set to 1; left panel ). One representative immunoblot is shown for each analysis, with indicated antibodies. The black arrowheads indicate endogenous eIF2α, and the white arrowheads indicate FLAG-tagged eIF2α. C The levels of ROS were measured in BT-549 cells stably expressing WT or O-GlcN-mut eIF2α after 48 h of ArgS treatment (n = 3). The ROS levels were quantified using CellROX™ and flow cytometry. D The relative cell recovery was monitored in BT-549 cells stably overexpressing WT or O-GlcN-mut eIF2α over a 5-day period, after 24 h of ArgS treatment and replacement with complete medium. The cell recovery was determined using ACP assays and the data were normalized to the values of Day 0, set to 1. E The cell migration was measured in BT-549 cells stably overexpressing WT or O-GlcN-mut eIF2α after 24 h of ArgS treatment and replacement with complete medium. The cell migration was determined by calculating the % confluency within the gap area and was imaged and quantified using Image J software at 0, 12, 24, and 30 h. Data are presented as mean ± s.e.m. and analyzed using Two-Way ANOVA followed by Tukey's multiple comparison test. Statistical significance was determined with * p < 0.05; ** p < 0.01; *** p < 0.001
Antigen Retrieval Buffer, supplied by Abcam, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tissue+antigen+recovery/pmc04367486-73-10-13?v=Abcam
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hcc cell lines hepg2  (ATCC)
99
ATCC hcc cell lines hepg2
The O-GlcNAcylation of eIF2α downregulates the antioxidant capacity, cell recovery and migratory ability of breast cancer cells in response to ArgS. A Immunoprecipitation coupled with immunoblot analysis was performed to measure the levels of GlcNAz-modified (O-GlcNAcylated) proteins in parental <t>HEK</t> <t>293T</t> cells (n = 3; left panel ). The O-GlcNAz-modified eIF2α level was calculated by normalizing the densitometric tracing of eIF2α signal to the H3 signal ( right panel ). The values were quantified using ImageLAB software and adjusted for background. B The same analysis was performed in WT and O-GlcN-mut, eIF2α overexpressing HEK <t>293</t> <t>T</t> cells (n = 2; left panel ). The O-GlcNAz-modified eIF2α level was calculated as described in A , and the ratio in each experimental condition was compared to the reference (WT + Arg, set to 1; left panel ). One representative immunoblot is shown for each analysis, with indicated antibodies. The black arrowheads indicate endogenous eIF2α, and the white arrowheads indicate FLAG-tagged eIF2α. C The levels of ROS were measured in BT-549 cells stably expressing WT or O-GlcN-mut eIF2α after 48 h of ArgS treatment (n = 3). The ROS levels were quantified using CellROX™ and flow cytometry. D The relative cell recovery was monitored in BT-549 cells stably overexpressing WT or O-GlcN-mut eIF2α over a 5-day period, after 24 h of ArgS treatment and replacement with complete medium. The cell recovery was determined using ACP assays and the data were normalized to the values of Day 0, set to 1. E The cell migration was measured in BT-549 cells stably overexpressing WT or O-GlcN-mut eIF2α after 24 h of ArgS treatment and replacement with complete medium. The cell migration was determined by calculating the % confluency within the gap area and was imaged and quantified using Image J software at 0, 12, 24, and 30 h. Data are presented as mean ± s.e.m. and analyzed using Two-Way ANOVA followed by Tukey's multiple comparison test. Statistical significance was determined with * p < 0.05; ** p < 0.01; *** p < 0.001
Hcc Cell Lines Hepg2, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tissue+antigen+recovery/pm32978504-61-2-13?v=ATCC
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tissue antigen  (Thermo Fisher)
99
Thermo Fisher tissue antigen
The O-GlcNAcylation of eIF2α downregulates the antioxidant capacity, cell recovery and migratory ability of breast cancer cells in response to ArgS. A Immunoprecipitation coupled with immunoblot analysis was performed to measure the levels of GlcNAz-modified (O-GlcNAcylated) proteins in parental <t>HEK</t> <t>293T</t> cells (n = 3; left panel ). The O-GlcNAz-modified eIF2α level was calculated by normalizing the densitometric tracing of eIF2α signal to the H3 signal ( right panel ). The values were quantified using ImageLAB software and adjusted for background. B The same analysis was performed in WT and O-GlcN-mut, eIF2α overexpressing HEK <t>293</t> <t>T</t> cells (n = 2; left panel ). The O-GlcNAz-modified eIF2α level was calculated as described in A , and the ratio in each experimental condition was compared to the reference (WT + Arg, set to 1; left panel ). One representative immunoblot is shown for each analysis, with indicated antibodies. The black arrowheads indicate endogenous eIF2α, and the white arrowheads indicate FLAG-tagged eIF2α. C The levels of ROS were measured in BT-549 cells stably expressing WT or O-GlcN-mut eIF2α after 48 h of ArgS treatment (n = 3). The ROS levels were quantified using CellROX™ and flow cytometry. D The relative cell recovery was monitored in BT-549 cells stably overexpressing WT or O-GlcN-mut eIF2α over a 5-day period, after 24 h of ArgS treatment and replacement with complete medium. The cell recovery was determined using ACP assays and the data were normalized to the values of Day 0, set to 1. E The cell migration was measured in BT-549 cells stably overexpressing WT or O-GlcN-mut eIF2α after 24 h of ArgS treatment and replacement with complete medium. The cell migration was determined by calculating the % confluency within the gap area and was imaged and quantified using Image J software at 0, 12, 24, and 30 h. Data are presented as mean ± s.e.m. and analyzed using Two-Way ANOVA followed by Tukey's multiple comparison test. Statistical significance was determined with * p < 0.05; ** p < 0.01; *** p < 0.001
Tissue Antigen, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tissue+antigen+recovery/pm16722994-36-15-53?v=Thermo+Fisher
Average 99 stars, based on 1 article reviews
tissue antigen - by Bioz Stars, 2026-06
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recoverin expression plasmid ptrec2  (ATCC)
92
ATCC recoverin expression plasmid ptrec2
The O-GlcNAcylation of eIF2α downregulates the antioxidant capacity, cell recovery and migratory ability of breast cancer cells in response to ArgS. A Immunoprecipitation coupled with immunoblot analysis was performed to measure the levels of GlcNAz-modified (O-GlcNAcylated) proteins in parental <t>HEK</t> <t>293T</t> cells (n = 3; left panel ). The O-GlcNAz-modified eIF2α level was calculated by normalizing the densitometric tracing of eIF2α signal to the H3 signal ( right panel ). The values were quantified using ImageLAB software and adjusted for background. B The same analysis was performed in WT and O-GlcN-mut, eIF2α overexpressing HEK <t>293</t> <t>T</t> cells (n = 2; left panel ). The O-GlcNAz-modified eIF2α level was calculated as described in A , and the ratio in each experimental condition was compared to the reference (WT + Arg, set to 1; left panel ). One representative immunoblot is shown for each analysis, with indicated antibodies. The black arrowheads indicate endogenous eIF2α, and the white arrowheads indicate FLAG-tagged eIF2α. C The levels of ROS were measured in BT-549 cells stably expressing WT or O-GlcN-mut eIF2α after 48 h of ArgS treatment (n = 3). The ROS levels were quantified using CellROX™ and flow cytometry. D The relative cell recovery was monitored in BT-549 cells stably overexpressing WT or O-GlcN-mut eIF2α over a 5-day period, after 24 h of ArgS treatment and replacement with complete medium. The cell recovery was determined using ACP assays and the data were normalized to the values of Day 0, set to 1. E The cell migration was measured in BT-549 cells stably overexpressing WT or O-GlcN-mut eIF2α after 24 h of ArgS treatment and replacement with complete medium. The cell migration was determined by calculating the % confluency within the gap area and was imaged and quantified using Image J software at 0, 12, 24, and 30 h. Data are presented as mean ± s.e.m. and analyzed using Two-Way ANOVA followed by Tukey's multiple comparison test. Statistical significance was determined with * p < 0.05; ** p < 0.01; *** p < 0.001
Recoverin Expression Plasmid Ptrec2, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tissue+antigen+recovery/pm11716523-46-10-17?v=ATCC
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recoverin expression plasmid ptrec2 - by Bioz Stars, 2026-06
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Image Search Results


The O-GlcNAcylation of eIF2α downregulates the antioxidant capacity, cell recovery and migratory ability of breast cancer cells in response to ArgS. A Immunoprecipitation coupled with immunoblot analysis was performed to measure the levels of GlcNAz-modified (O-GlcNAcylated) proteins in parental HEK 293T cells (n = 3; left panel ). The O-GlcNAz-modified eIF2α level was calculated by normalizing the densitometric tracing of eIF2α signal to the H3 signal ( right panel ). The values were quantified using ImageLAB software and adjusted for background. B The same analysis was performed in WT and O-GlcN-mut, eIF2α overexpressing HEK 293 T cells (n = 2; left panel ). The O-GlcNAz-modified eIF2α level was calculated as described in A , and the ratio in each experimental condition was compared to the reference (WT + Arg, set to 1; left panel ). One representative immunoblot is shown for each analysis, with indicated antibodies. The black arrowheads indicate endogenous eIF2α, and the white arrowheads indicate FLAG-tagged eIF2α. C The levels of ROS were measured in BT-549 cells stably expressing WT or O-GlcN-mut eIF2α after 48 h of ArgS treatment (n = 3). The ROS levels were quantified using CellROX™ and flow cytometry. D The relative cell recovery was monitored in BT-549 cells stably overexpressing WT or O-GlcN-mut eIF2α over a 5-day period, after 24 h of ArgS treatment and replacement with complete medium. The cell recovery was determined using ACP assays and the data were normalized to the values of Day 0, set to 1. E The cell migration was measured in BT-549 cells stably overexpressing WT or O-GlcN-mut eIF2α after 24 h of ArgS treatment and replacement with complete medium. The cell migration was determined by calculating the % confluency within the gap area and was imaged and quantified using Image J software at 0, 12, 24, and 30 h. Data are presented as mean ± s.e.m. and analyzed using Two-Way ANOVA followed by Tukey's multiple comparison test. Statistical significance was determined with * p < 0.05; ** p < 0.01; *** p < 0.001

Journal: Journal of Biomedical Science

Article Title: Extracellular arginine availability modulates eIF2α O-GlcNAcylation and heme oxygenase 1 translation for cellular homeostasis

doi: 10.1186/s12929-023-00924-4

Figure Lengend Snippet: The O-GlcNAcylation of eIF2α downregulates the antioxidant capacity, cell recovery and migratory ability of breast cancer cells in response to ArgS. A Immunoprecipitation coupled with immunoblot analysis was performed to measure the levels of GlcNAz-modified (O-GlcNAcylated) proteins in parental HEK 293T cells (n = 3; left panel ). The O-GlcNAz-modified eIF2α level was calculated by normalizing the densitometric tracing of eIF2α signal to the H3 signal ( right panel ). The values were quantified using ImageLAB software and adjusted for background. B The same analysis was performed in WT and O-GlcN-mut, eIF2α overexpressing HEK 293 T cells (n = 2; left panel ). The O-GlcNAz-modified eIF2α level was calculated as described in A , and the ratio in each experimental condition was compared to the reference (WT + Arg, set to 1; left panel ). One representative immunoblot is shown for each analysis, with indicated antibodies. The black arrowheads indicate endogenous eIF2α, and the white arrowheads indicate FLAG-tagged eIF2α. C The levels of ROS were measured in BT-549 cells stably expressing WT or O-GlcN-mut eIF2α after 48 h of ArgS treatment (n = 3). The ROS levels were quantified using CellROX™ and flow cytometry. D The relative cell recovery was monitored in BT-549 cells stably overexpressing WT or O-GlcN-mut eIF2α over a 5-day period, after 24 h of ArgS treatment and replacement with complete medium. The cell recovery was determined using ACP assays and the data were normalized to the values of Day 0, set to 1. E The cell migration was measured in BT-549 cells stably overexpressing WT or O-GlcN-mut eIF2α after 24 h of ArgS treatment and replacement with complete medium. The cell migration was determined by calculating the % confluency within the gap area and was imaged and quantified using Image J software at 0, 12, 24, and 30 h. Data are presented as mean ± s.e.m. and analyzed using Two-Way ANOVA followed by Tukey's multiple comparison test. Statistical significance was determined with * p < 0.05; ** p < 0.01; *** p < 0.001

Article Snippet: Human triple-negative breast cancer BT-549, MDA-MB-231, Hs578T, MDA-MB-468, and MDA-MB-435, human estrogen receptor- and progesterone receptor-positive breast cancer MCF7, and human embryonic kidney (HEK) 293T (HEK293 cells with SV40 T-antigen) cells were originally purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Cell Recovery, Immunoprecipitation, Western Blot, Modification, Software, Stable Transfection, Expressing, Flow Cytometry, Migration, Comparison